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The Camspec M550 Double Beam Scanning UV/Vis Spectrophotometer

Camspec M550 Spectrophotometer

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Main Features and Benefits

The M550 is a high performance, true double beam UV/Visible spectrophotometer with parallel sample and reference beams and is supplied with two x 10mm precision cell holders as standard.

Two silicon photodiodes are used for measuring the two beams simultaneously, thereby ensuring the measurement accuracy and stability. The reference beam is especially useful for measuring samples where the reference solution changes with time.

The M550 uses an easy-to-replace tungsten-halogen lamp and a pre-aligned, long-life Hamamatsu deuterium lamp.

The specification is as expected from a top-of-the-range instrument designed for both research and routine use.

User Interface

The user interface is almost the same as that of the popular M501 single beam scanning spectrophotometer and includes:

  • Basic Mode - Absorbance, %T, Concentration
  • Quantitative Mode - Concentration calibration curves
  • Wavelength Scanning Mode
  • Kinetics Mode - Including time/drive
  • DNA/Protein Analysis Mode
  • Multi-wavelength Mode
  • Performance Validation Mode

The M550 stores up to 50 methods / results. In Basic Mode, the result is continuously displayed, i.e. no need to "press to read".

PC Control

The M550 has a bi-directional RS232C serial interface for transfer of results to a PC and for PC control of the M550. Comprehensive Camspec Application consumables is available, together with a number of small PC programs dedicated to specialist tasks.

Accessories

An extensive range of sample compartment accessories (which can also be used with the M501) is available and includes:

  • Test tube V-type holder (100mm or 120mm diameter tubes)
  • Adjustable micro-cell holder
  • 4 x 10mm manual cell changer
  • 4-cell holder for 5 to 50mm cells
  • 4-cell holder for 100mm cells
  • 100mm cylindrical cell holder
  • Thermostatted single cell holder (requires external water bath)
  • Automatic 6-position carousel cell changer (M550 only)
  • Sipper system, processor controlled
  • Peltier system for 10mm cells, 15 to 40°C
  • Combined peltier / sipper system
  • Cell stirrer for 10mm cells
  • Specular reflectance accessory (5° incident angle)
  • Integrating sphere for diffuse transmittance and reflectance applications
  • Calibration standards for wavelength, absorbance, stray light - traceable to NPL
  • Pharmacopoeia compliance certificated test set - UKAS accredited

Pharmaceutical Analysis

The M550 is European Pharmacopoeia 5.2 compliant and passes the following tests:

  • Potassium Chloride solution stray light test
  • Toluene in Hexane solution resolution test
  • Holmium Perchlorate solution wavelength test
  • Potassium Dichromate solution absorbance test

The M550 may be controlled by Camspec Panorama software which is 21 CFR Part II compliant. This means that it can be used for pharmaceutical analysis requiring FDA (US Food and Drug Administration) approval.

Camspec is an ISO 9001:2000 company and all instruments are individually checked using wavelength and absorbance standards calibrated by the National Physical Laboratory.

The M550 fully complies with CE requirements for safety and ESD / EMC emission and susceptibility.


SPECIFICATIONS

Display 1/4 VGA - 320 x 240 pixels, backlit LCD
Light Sources Tungsten-Halogen and Deuterium
Monochromator Littrow type with 1200 lines/mm grating
Detector Two Silicon Photodiodes
Wavelength Range 190 - 1100nm
Wavelength Accuracy ± 0.3nm
Wavelength Resolution 0.1nm
Wavelength Repeatability ± 0.05nm
Bandpass 1.8nm
Photometric Range -0.3 to 3.0A, 0 to 200%T and 0 to 9999 Conc
Photometric Accuracy Better than 1% @ 0.5A, 1A and 2A
Scan Speed Up to 2500nm / min (return 3000nm / min)
Noise Less than 0.0001A @ 500nm 0A
Zero Drift Less than 0.001A per hour after warm-up
Baseline Flatness ± 0.002A
Stray Light (ASTM) Less than 0.05%T @ 220nm and 340nm
Printer Interface Centronic parallel - for A4 HP and Epson Printers
Computer Interface Bi-directional RS232C for PC control
Power Requirements 110/120V AC, 220/230V AC, 50/60Hz, 110VA
Dimensions (W) 630mm x (D) 410mm x (H) 280mm
Weight 24 Kgs

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